MR202106
Quantitative imaging analysis of nanoparticles and dissolved forms using laser ablation-single particle-ICP-mass spectrometry
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- Quantitative imaging analysis of nanoparticles and dissolved forms using laser ablation-single particle-ICP-mass spectrometry
Shuji Yamashita, Kumiko Ogawa, Takafumi Hirata
Received: August 31, 2021
Accepted: November 18, 2021
Released online: January 06, 2022
Abstract
Laser ablation-single particle-ICP-mass spectrometry (LA-spICP-MS) was applied to define the size, position of silver nanoparticles (Ag NPs), and the concentration of ionic Ag (dissolved Ag) on a frozen section of mouse liver (6 hours after intraperitoneal administered 60 nm Ag NPs (0.2 mg per mouse)). For the accurate size calibration of Ag NPs and quantitative analysis of ionic Ag, a cellulose filter paper doped with Ag NPs suspension and a custom-made photocurable resin reference material containing ionic Ag were prepared in this study. From the imaging results of liver sample, preferential accumulation of the Ag NPs in certain regions was observed. Ionic Ag was also accumulated at regions where Ag NPs are. This suggests that there is a possible contribution of dissolution of Ag NPs through cell activity. This is supported by the detection of small Ag NPs (8–20 nm). The simultaneous imaging analyses of both Ag NPs and ionic Ag can become a useful tool to understand the mechanism of incorporation or metabolism of the NPs.